Fig. 7

Correlation heatmap of colorectal bacteriome metabolome using Distance correlation test as similarity method. 10% of reads were removed as variance filter based on inter-quartile range, reads with counts less than 4 were also removed as abundance filter. Data scaled via autoscaling as normalization. Different studied groups were used as primary metadata (fixed effects). Age, BMI, and sex of the participants were used as covariates and accounted for in the statistics extracted for the primary metadata. MaAsLine2 is used for microbiome comparison analysis and Limma is used for metabolomics data. Features with a p-value less than 0.01 were considered significant. Significant correlations are highlighted with asterisks. The correlation threshold was set to more than 0.35. The color indicates the correlation calculated by the distance test as a similarity method. Comparisons were done between the healthy (H) vs. adenomatous polyp (AP) group (A), and the H vs. cancer tumor (CT) group (B) at the species taxonomic level with their identified metabolites according to their relative abundances