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Fig. 8 | Annals of Clinical Microbiology and Antimicrobials

Fig. 8

From: Novel strategies for vancomycin-resistant Enterococcus faecalis biofilm control: bacteriophage (vB_EfaS_ZC1), propolis, and their combined effects in an ex vivo endodontic model

Fig. 8

Characterization of EF/14 biofilm morphology and viability following irrigation treatments in ex vivo model of root dentin slices. A Sterilized blank group without biofilm, while (B) contained a four-week-old biofilm without any treatment. (C- G) underwent different irrigated treatments: (C) was treated with 2% Sodium Hypochlorite, (D) with 0.9% saline, (E) with propolis, (F) with phage vB_EfaS_ZC1, and (G) with a combination of phage vB_EfaS_ZC1 and propolis. These groups are represented in the corresponding figure. B1-G1 Qualitative analysis was observed by FESEM images of the EF/14 biofilm after 10 min of different irrigation treatments. B2–G2 Quantitative analysis by CLSM illustrating EF/14 biofilms (green: live bacteria; red: dead bacteria) on dentin slices after 10 min of different irrigation treatments. H Box plots demonstrate the percentage of live bacterial cells under different irrigation treatments. The median and interquartile range (n = 6) are represented for each group, Statistical significance is indicated by *** for P < 0.001, and ns indicates no significant difference.

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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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